Aqueous garlic extract protects against sepsis induced toxicity in pulmonary and ileal tissues

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Published: Apr 15, 2016
Keywords:
Ileal injury Pulmonary injury Septic Thromboplastic activity Oxidative stress Rat

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Sevil Ozkan
Dept. of Internal Medicine, Haydarpasa Numune Research Hospital, Istanbul, Turkey
Tugba Tunalı Akbay
Dept. of Medical Biochemistry, Marmara University, Faculty of Medicine, Istanbul, Turkey
Selin Sakarcan
Dept. of Microbiology, Temple University, Faculty of Medicine, Pennsylvania, USA
Emre Gunay
Dept. of General Surgery, Haydarpasa Numune Research Hospital, Istanbul, Turkey
Goksel Sener
Dept. of Pharmacology, Marmara University, Faculty of Pharmacy, Istanbul, Turkey

Abstract

Objective: Based on the potent antioxidant effects of aqueous garlic extract (AGE), the present study was designed to characterize the potential of AGE to modify blood coagulation parameters as well as and pulmonary and ileal injury in septic rats. Sepsis was induced using the caecal ligation and perforation (CLP) method.


Methods: Twenty-four hours after sepsisinduction, rats were decapitated and trunk blood was collected for the measurement of platelet counts, fibrinogen, prothrombin time, activated partial thromboplastin time and D-dimer levels. Then, pulmonary and ileal tissue samples were immediately obtained and stored at – 70oC for malondialdehyde (MDA), glutathione, myeloperoxidase (MPO) and superoxide dismutase (SOD) activity assays.


Results: Sepsis was associated with a decrease in platelet count and fibrinogen and an increase in APTT and INR. It also caused a significant decrease in GSH levels and SOD activity in both pulmonary and ileal tissue samples. On the other hand, AGE treatment in rats with CLP caused significantly augmented the level of these antioxidants. As a result of CLP induction increased MPO activity and MDA levels and decreased thromboplastic activity were reversed with AGE treatment.


Conclusion: AGE treatment, through its antioxidant effects, protects against oxidative pulmonary and ileal injury and normalizes the impaired coagulation in sepsis. 

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How to Cite
Ozkan, S. ., Akbay , T. T., Sakarcan, S. ., Gunay, E. ., & Sener, G. . (2016). Aqueous garlic extract protects against sepsis induced toxicity in pulmonary and ileal tissues. Medical Science and Discovery, 3(4), 192–199. Retrieved from https://medscidiscovery.com/index.php/msd/article/view/124
Issue
Vol. 3 No. 4 (2016): Medical Science and Discovery
Section
Research Article

https://creativecommons.org/licenses/by-nc/4.0/

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