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In this review, we aimed to emphasize the importance of immobilized enzymes for human health in shed light on recent literature. In addition to our clinical experiences, some literature studies on immobilized enzymes were evaluated. The immobilized enzymes bind to a specific region physically by using mediator enzymes and shows catalytic activities repeatedly and continuously without losing their catalytic activities. In other words, enzyme immobilization is the trapping or binding of the insoluble form of the enzyme or the carrier agent to itself. Compared to free enzymes in solution, immobilized enzymes are tougher and more resistant to environmental changes. Some immobilized enzymes are also used in various industries. Immobilization techniques are generally used in industrial processes, diagnostics, bio-affinity chromatography and biosensors applications. As recently, immobilized enzymes have begun to be used in dissolving blood clots and clearing wounds.
Stryer, L. Biochemistry; 1995, Freeman, New York.
Tosa T, Mori T, Fuse N, and Chibata I. Studies on continuous enzyme reactions. I. Screening of carriers for preparation of water-insoluble aminoacylase. Enzymologia. 1966; 31(4):214-24.
Guibault, GG, Kauffmann J M, and Patriarche J. Immobilized Enzyme Electrodes as Biosensors. In: Protein Immobilization. Fundamentals and Applications (Taylor, R. F., ed.), Marcel Dekker, New York, NT. 1991; pp. 209–262.
Taylor R F. Immobilized Antibody and Receptor Based Biosensors. In: Protein Immobilization. Fundamentals and Applications (Taylor, R. F., ed.), Marcel Dekker, New York, NY. 1991; pp. 263–303.224.
Savran A, Alkan S, Demir H, and H Ceylan. Applicaton of Natural Kaolin as Support for The Immobilization of Catalase from Bovine Liver, Asian Journal of Chemistry. 2006; 18, 413-418.
Telefoncu A. Enzimoloji. Yüksek Lisans Yaz okulu. 1997; 21-27 Eylül 1997.Kuşadası, Aydın, Türkiye.446 s.
Phutela U, Dhuna V, Sandhu S, Chadha BS. Pectinase and Polygalacturonase Production by a Thermophilic Aspergillus fumigatus Isolated from Decomposting Orange Peels. Brazilian Journal of Microbiology. 2005; 36:63-69.
Patıl S R, Dayanand A. Exploration of Regional Agrowastes for the Production of Pectinase by Aspergillus niger. Food Technol. Biotechnol. 2006; 44.289-292.
Botella C, Dıaz A, Ory I, Webb C, Blandıno A. Xylanase and Pectinase Production by Aspergillus awamori on Grape Pomace in Solid State Fermentation. Process Biochemistry. 2006; 42:98-101.
Katchalski-Katzir E. Immobilized enzymes: Learning from past successes and failures. Trends Biotechnol. 1993; 11, 471–478.
Beatriz M B, and Francisco Batista-V. Immobilization of Enzymes. Methods in Biotechnology: Immobilization of Enzymes and Cells. 2017; Second Edition Edited by: J. M. Guisan © Humana Press Inc., Totowa, NJ.
Burns RG. Soil Science Society of America, W. 1986; 439.
Alkan S, Gür T, Gür A, Uruc H and Demir H. Immobilization of Catalase via Adsorption onto Natural and Modifed Montmorillant Analsim-Clay. Polish. Journal of Chem. 2009; 83, 2089-2095.
Baileey JE, and Ollis DF. Applied Enzyme Catalysis, McGraww-Hill International. 1986; Singapore, p.180.
Kennedy JF, Melo EHM. Immobilized enzymes and Cells. Chem.Eng.Prog. 1990; 81.
Gabel D, Steinberg I, and Katchalski-Kazir E. Changes in conforma- tion of insolubilized trypsin and chymotrypsin, followed by fluorescence. Biochemistry. 1971; 10, 4661–4669.
Koch-Schmidt A and Mosbach K. Studies on conformation of soluble and immobilized enzymes using differential scanning calorimetry. 1. Thermal stability of nicotinamide adenine dinucleotide dependent dehydrogenases. Biochemistry. 1977a; 16, 2101–2105.
Koch-Schmidt A, and Mosbach K. Studies on conformation of soluble and immobilized enzymes using differential scanning calorimetry. 2. Specific ac- tivity and thermal stability of enzymes bound weakly and strongly to Sepharose CL 4B. Biochemistry. 1977b; 16, 2105–2109.
Gray CJ, Weissenborn MJ, Eyers E, Flitsch SL. "Enzymatic reactions on immobilised substrates". Chemical Society Reviews. 2013; 42 (15): 6378.
Mitz MA.. New soluble active derivatives of an enzyme as a model for study of cellular metabolism. Science. 1956; Vol.123; pp.1076–1077.
Sungur S, and Yıldırım Ö.. Batch and Contınuous Hydrolysıs of Lactose Usıng β-Galactosıdase Immobilized on Gelatın-CMC. Department of Chemistry and Biology, Faculty of Science, Ankara University, Polym-Plast. Technol. ENG.,1999; Vol.38; pp.821–829.
SuY, LiW, ZhuW, YuR, FeiR, WenT, CaoY, and Qiao D. Characterization of xylose reductase from Candida tropicalis immobilized on chitosan bead. African Journal of Biotechnology. 2010; Vol.9(31); pp.4954–4965.
Bourget L, and Chang T M. Artificial cell-microencapsulated phenylalanine ammonia-lyase. Appl. Biochem. Biotechnol. 1984; 10: 57 – 59.
Ateş S, Doğan N S. Properties of Immobilized Phenylalanine Ammonia Lyase and Investigation of its Use for the Prediagnosis of Phenylketonuria. Türk Biyokimya Dergisi. 2010; 35: 58–62.
Dogan NE. Utılızatıon Of Immobılızed Enzyme In Dıagnosıs of Phenylketonurıa. Gazı Unıversıty Instıtute Of Scıence And Technology. 2008; (M. Sc. Thesis). Ankara.