Is traditional herbal mix composition effective on wound healing? antique oil and wound healing

Main Article Content

Serap Gokce Eskin
Yucel Basimoglu Koca
Serdal Ogut


Objective: Wound healing is a dynamic process that includes biochemical and physiological phenomena. Studies confirm that plants are used in wound healing to a great extent.  Antique oil (HBX 2371) has been produced as a traditional herbal mixed. This herbal mixture contains sesame oil, thyme oil, olive leaf, fig seed, grape seed, turmeric, and cinnamon. We evaluated traditional plant mixed oil ( Antique oil) for its wound-healing activity using an excision wound model in rats.

Methods: The study used a preclinical, in vivo experimental, and analytical design. In the study, a total of 21 Sprague-Dawley rats, including 7 as experimental group, 7 as negative-control group, and 7 as positive-control group, were used. After the ischemic wound was created in experimental animals, the research data relating to the histochemical changes and biochemical parameters of the wound healing parameters were collected

Results: A to research in experimental group fibroblast count, collagen density, fat cells, epithelization scores higher than the other groups and inflammatory cell density lower than the others. According to the results of the study, the highest TAC value (3.94 ± 0.21) was determined in the experimental group and results showed that antique oil administration decreased the TOC value.

Conclusion: In conclusion, it was observed that antique oil prevented the wound changes induced by the incision, increased the repair of the epidermal and dermal structure in a short time, increased the antioxidant level, and decreased the oxidation level. Clinical studies are recommended.


Download data is not yet available.

Article Details

How to Cite
Eskin, S. G., Koca, Y. B., & Ogut, S. (2021). Is traditional herbal mix composition effective on wound healing?. Medical Science and Discovery, 8(1), 29-38.
Research Article


1. Ellis S, Lin, E, Tartar D. Immunology of Wound Healing. Curr Derm Rep.2018; 7:350–358.

2. Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. Wound Healing: A Cellular Perspective. Physiol Rev.2019; 99: 665–706. doi: 10.1152/physrev.00067.2017

3. Singh S, Young A, Mcnaught CE. The physiology of wound healing. Surgery (Oxford). 2017;35(9): 473-477

4. Barrientos S, Brem H, Stojadinovic O, Tomic-Canic M. Clinical application of growth factors and cytokines in wound healing. Wound Repair Regeneration. 2014; 22: 569–578.

5. Quing C. The molecular biology in wound healing & non-healing wound. Chinese Journal of Traumatology. 2017;20(4):189-193

6. Sorg H, Tilkorn D, J, Hager S, Hauser J, Mirastschijski U: Skin Wound Healing: An Update on the Current Knowledge and Concepts. Eur Surg Res 2017;58:81-94. doi: 10.1159/000454919

7. Alam G, Singh MP, Singh A. Wound healing potential of some medicinal plants. International Journal of Pharmaceutical Sciences Review and Research. 2011; 9(1): 136-145.

8. Abdou HM, Hussien HM, Yousef MI. Deleterious effects of cypermethrin on rat liver and kidney: protective role of sesame oil. J Environ Sci Heal B.2012; 47(4): 306-14.

9. Kumar M. Hepatoprotective activity of Sesamum Indicum Linn. against CCl4-induced hepatic damage in rats. Int J Pharm Biol Arch.2011; 2(2): 710-5.

10. Kowalczyk A, Przychodna M, Sopata S, Bodalska A, Fecka I. Thymol and Thyme Essential Oil—New Insights into Selected Therapeutic Applications. Molecules. 2020;25:4125.

11. Salehi B, Mıshra AP, Shukla I, Sharifi-Rad M, ContrerasMM, Segura ‐ Carretero A, Fathi H, Nasrabadi NN, Kobarfard F, Sharifi-Rad J. Thymol, thyme, and other plant sources: Health and potential uses. Phytotheraphy Research. 2018; 32(9): 1688-1706

12. Yaman S, Özdemir Z , Şit M , Özer B, Çatal O. Effects of Oregano Oil Carvacrol on Human Health. SETSCI Conference Indexing System. 2018; (2): 391-392.

13. Coban EÖ, Patır B. Use of Some Spices and Herbs Antioxidant Affected in Foods. Electronic Journal of Food Technologies. 2010; 5(2): (7-19).

14. Mariam AK. The effect of oıl extract of thymus vulgarıs on full thıckness ıncısıon of wound healıng in rabbits. Bas.J.Vet.Research. 2015; 14(2): 103-109.

15. Panah G K, Hesaraki S and Farahpour MR. Histopathological evaluation of thymus vulgaris on wound healing. Indian Journal of Fundamental and Applied Life Sciences. 2014; 4 (S4): 3538-3544.

16. Tümen G, Malyer H, Başer KHC, Özaydın S. Plant used in Anatolia for wound healing. Proceedings of the IVth International Congress of Ethnobotany (ICEB 2005). 2006; 217-221.

17. Hemmati AA, Aghel N, Rashidi I, Gholampur-Aghdami A. Topical grape (Vitis vinifera) seed extract promotes repair of full thickness wound in rabbit.Int Wound Journal. 2011; 8:514–520.

18. Nayak SD, Dan Ramdath DB, Marshall JR, Isitor GN, Eversley M, Xue S, Shi J. Wound-healing activity of the skin of the common grape (vitis vinifera) variant, cabernet sauvignon. Phytotherapy Research.2010; 24: 1151–1157 DOI: 10.1002/ptr.2999

19. Ma H, Quan Zhou Q, Chang J, Wu C. ACS Nano. 2019; 13 (4): 4302-4311. DOI: 10.1021/acsnano.8b09496

20. Nakilcioğlu Taş E. Biochemical Characterization of Fig (Ficus carica L.) Seeds. Journal of Agricultural Sciences.2019;25: 232-237.

21. Orak C. Possıble effects of fıg (Ficus carica) seed oıl on ıschemıareperfusıon damage, resultıng from experımental mesenterıc arterıal ıschemıa ın small ıntestıne. Aydin Adnan Menderes University Health Sciences Institute of Physiology.(Medical) Program, Doctoral Thesis, Aydin, 2020.

22. Eteraf-Oskouei T, Allahyari S, Akbarzadeh-Atashkhosrow A, Delazar A, Pashaii M, Gan Sh, Najafi M. Methanolic Extract of Ficus carica Linn. Leaves Exerts Antiangiogenesis Effects Based on the Rat Air Pouch Model of Inflammation. Evidence-Based Complementary and Alternative Medicine. 2015. Article ID 760405 |

23. Moghadamtousi SZ, Kadir HA, Hassandarvish P, Tajik H, Abubakar S, Zandi K. A review on antibacterial, antiviral, and antifungal activity of curcumin. Biomed Res Int. 2014; 1–12. doi:

24. Guimarães AF,Vinhas ACA, Gomes AF, Souza, LH, Krepsky PB. Essentıal oıl of Lurcuma Longa L. Rhızomes chemıcal composıtıon, yıeld varıatıon and stabılıty. Química Nova.2020;43(7): 909-913.

25. Farahpour MR, Habibi M. Evaluation of the wound healing activity of an ethanolicextract of ceylon cinnamon in mice. Veterinarni Medicina.2012; 57(1): 53–57.

26. Faix S, Faixová z, Plachá I, Koppel J. Effect of cinnamomum zeylanicum essential oil on antioxidative status in broiler chickens. Acta Vet. Brno.2009; 78: 411-417; doi:10.2754/avb200978030411.

27. Koca U, Süntar I, Akkol EK, Yilmazer D, Alper M. Wound repair potential of Olea europaea L. leaf extracts revealed by in vivo experimental models and comparative evaluation of the extracts' antioxidant activity. J Med Food. 2011;14(1-2):140-6. doi: 10.1089/jmf.2010.0039.

28. Samancıoglu S, Esen A, Ercan G, Mansoub NH, Vatansever S, İnce İ. A new dressing material in diabetic wounds: Wound healing activity of oleuropein-rich olive leaf extract in diabetic rats. Gaziantep Medical Journal 2016;22(1):14-21 • DOI: 10.5578/GMJ.27960

29. Gurbuz M, Ogut S. 2020. Antidiabetic effect of olive leaf extract on streptozotocin-induced diabetes mellitus in experimental animals. Nutr Hosp. 2020:37(5): 1012-1021.

30. Guo, Z., Jia, X., Zheng, Z. et al. Chemical composition and nutritional function of olive (Olea europaea L.): a review. Phytochem Rev.2018; 17: 1091–1110

31. Festing MF, Altman DG. Guidelines for the design and statistical analysis of experiments using laboratory animals. ILAR J. 2002;43(4):244–258.

32. Gokce S, Tureyen, Gokcimen A. Effects of hypericum perforatum, clinoptilolite and hydrocolloid dressing on wound healing in diabetic rats. Wound Medicine. 2018; 22: 14-22

33. Bancroft JD, Cook HC. Manual of Histological Techniques and Their Diagnostic Application. Churchill livingstone. London, 1994.

34. Magalhaes, L.s.M., et al., Methodological aspects about in vitro evaluation of antioxidant properties. Analytica chimica acta, 2008; 613(1): 1-19.

35. Erel O, A novel automated method to measure total antioxidant response against potent free radical reactions. Clinical biochemistry, 2004; 37(2):112-119.

36. Ogut S, Kucukoner E, Gultekin F, Gurbuz N. (2014). Study of long-term pesticide application amongst agricultural workers: total antioxidant status, total oxidant status and acetylcholinesterase activity in blood. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. DOI 10.1007/s40011-013-0291-6.

37. Erel Ö. A new automated colorimetric method for measuring total oxidant status, Clinical Biochemistry. 2005;38:1103–111.

38. Sezer K, Keskin M. Serbest oksijen radikallerinin hastalıkların patogenezisindeki rolü. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi 2014, 28 (1), 49 – 56.

39. Sen CK, Packer L, Thiol homeostasis and supplements in physical exercise. The American journal of clinical nutrition, 2000; 72(2): 653-69.

40. Omma A, Sandikci SC, Kucuksahin O, Alisik M, Erel O, Can the Thiol/Disulfide Imbalance Be a Predictor of Colchicine Resistance in Familial Mediterranean Fever? Journal of Korean medical science, 2017;32, 10: 1588-94.

41. Erel O, Neselioglu S . A novel and automated assay for thiol/disulphide homeostasis. Clinical Biochemistry 2014; 47(18): 326-332.