Examination of Impact of Di(2-ethylhexyl) Phthalate and Dibutyl Phthalate on Rat Internal Organs by Scanning Acoustic Microscopy and Inductively Coupled Plasma Optical Emission Spectroscopy

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Bukem Tanoren
Gurcan Albeniz
Mufide Aydogan Ahbab
Leyla Turker Sener
Işıl Albeniz
Fatma Ates Alkan
Nural Pastaci Ozsobaci
Berzem Selcuk
Mehmet Burcin Unlu

Abstract

Objective: Phthalates, despite their endocrine disrupting effects, are widely used as plastifiants. Environmental exposure of phthalates was demonstrated to cause fetal death and reproductive toxicity in human beings, as well as in laboratory animals. However, underlying mechanisms are not clear.


Material and Methods: Here, we examine the impact of di(2-ethylhexyl) phthalate (DEHP) and dibutyl phthalate (DBP)on rat lungs, brain and heart by scanning acoustic microscopy (SAM) and inductively coupled plasma optical emission spectroscopy (ICP-OES). First, we evaluate tissues of mother rats and we show that the acoustic impedance values oftissues of DEHP and DBP delivered rats differ from those of tissues of the control rat. Then, element level analyses withinthese tissues are done and element levels within tissues of DEHP and DBP delivered pregnant rats are found to be higherthan those within tissues of the control pregnant rat. We then evaluate the tissues of offspring female rats.


Results: It is shown that acoustic impedance values of tissues of offspring rats of DEHP and DBP delivered mother rats are higher than those of tissues of the control offspring rats of the control mother rat. Besides, element analysis revealshigher element levels in the tissues of offspring rats of DEHP and DBP delivered mother rats.


Conclusion: Therefore, we can conclude that phthalates cause structural and functional changes within rat internal organs such as lungs, brain and heart. In summary, both modalities are confirmatory in a way that tissues of DEHP and DBP exposed pregnant rats and their offspring rats are differentiated by different acoustic impedance values obtained by SAM and higher element levels specified by ICP-OES.

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How to Cite
Tanoren, B., Albeniz, G., Ahbab, M. A. ., Sener , L. T., Albeniz, I. ., Alkan, F. A. ., Ozsobaci, N. P. ., Selcuk, B., & Unlu, M. B. (2021). Examination of Impact of Di(2-ethylhexyl) Phthalate and Dibutyl Phthalate on Rat Internal Organs by Scanning Acoustic Microscopy and Inductively Coupled Plasma Optical Emission Spectroscopy. Medical Science and Discovery, 8(4), 275–282. https://doi.org/10.36472/msd.v8i4.534
Section
Research Article
Received 2021-04-11
Accepted 2021-04-25
Published 2021-04-25

References

Neubert D. Vulnerability of the Endocrine System to Xenobiotic Influence. Regul Toxicol Pharmacol. 1997; 26: 9–29.

Sharpe RM. Natural and anthropogenic environmental oestrogens: the scientific basis for risk assessment; environmental oestrogens and male ınfertility. Pure Appl Chem. 1998; 70(9): 1685–1701.

Pryor JL, Hughes C, Foster W, et al. Critical windows of exposure for children’s health: the reproductive system in animals and humans. Environ Heal Perspectives 2000; 108: 491–503.

Sweeney T. Is exposure to endocrine distrupting compounds during fetal/postnatal development affecting the reproductive potential of farm animals? Domest Animal Endocrinol. 2002; 23: 203–9.

Latini G, Felice CD, Verrott A. 2004. Plasticizers, infant nutrition and reproductive health. Reproductive Toxicol. 2004; 19: 27–33.

Latini G. The potential hazards of exposure to di-(2-ethylhexyl)-phthalate in babies: A review. Biol Neonate. 2000; 78: 269–76.

Saiko I, Onuki A, Seto H. Determination of organic phosphate triesters in indoor and outdoor air. Aerosol Res. 2001; 16: 209–16.

Okubo T, Suzuki T, Yokoyama Y, et al. 2003. Estimation of estrogenic and anti-estrogenic activites of some phthalate diesters and monoesters by MCF-7 cell proliferation assay in vitro. Biol Pharm Bull. 2003; 26: 1219–24.

Hellwig J, Freudenberger H, Jackh R. Differential prenatal toxicity of branched phthalate esters in rats. Food Chem Toxicol. 1997; 35: 501–12.

Park JD, Habeebu SSM, Klaassen CD. Testicular toxicity of di-(2-ethylhexyl)phthalate in young sprague-dawley rats. Toxicology 2002; 171: 105–15 .

Li XJ, Jiang L, Chen L, et al. Neurotoxicity of dibutyl phthalate in brain development following perinatal exposure: A study in rats. Environ Toxicol Pharmacol. 2013; 36: 392–402.

Lin H, Yuan K, Li L, et al. In utero exposure to diethylhexyl phthalate affects rat brain development: A behavioral and genomic approach. Int J Environ Res Public Heal. 2015 12(11): 392–402.

Choi YJ, Ha KH, Kim DJ. Exposure to bisphenol a is directly associated with inflammation in healthy korean adults. Environ Sci Pollut Res 2016; 24(1): 284–90.

Robinson L, Miller R. The impact of bisphenol a and phthalates on allergy, asthma, and immune function: a review of latest findings. Curr Environ Heal Reports 2015; 2(4): 379–87 .

Nam SY, Ricles LM, Suggs LJ, et al. Imaging strategies for tissue engineering applications. Tissue Eng. 2015; 21(1): 88–102.

Saijo Y, Ohashi T, Sasaki H, et al. Application of scanning acoustic microscopy for assessing stress distribution in atherosclerotic plaque. Annals Biomed Eng. 2001; 29: 1048–53.

Saijo Y, Miyakawa T, Sasaki H, et al. Acoustic properties of aortic aneurysm obtained with scanning acoustic microscopy. Ultrasonics 2004; 42: 695–98.

Saijo Y, Filho ES, Sasaki H, et al. Ultrasonic tissue characterization of atherosclerosis by a speed-of-sound microscanning system. IEEE Transactions on Ultrason. Ferroelectr Freq Control 2007; 54(8):1571–77.

Miura K, Yamamoto S. Pulmonary imaging with a scanning acoustic microscope discriminates speed-of-sound and shows structural characteristics of disease. Lab Investig. 2012; 92: 1760–65.

Miura K, Nasu H, Yamamoto S. Scanning acoustic microscopy for characterization of neoplastic and inflammatory lesions of lymph nodes. Sci Reports 2013; 3: 1255.

Brewin MP, Srodon PD, Greenwald SE, et al. Carotid atherosclerotic plaque characterization by measurement of ultrasound sound speed in vitro at high frequency, 20 MHz. Ultrasonics 2014; 54: 428–41.

Miura K, Egawa Y, Moriki T, et al. Microscopic observation of chemical modification in sections using scanning acoustic microscopy. Pathol Int. 2015; 65 (7): 355–66.

Akhtar R, Cruickshank JK, Zhao X, et al. A pilot study of scanning acoustic microscopy as a tool for measuring arterial stiffness in aortic biopsies. Artery Res. 2016; 13: 1–5.

Saijo Y, Hozumi N, Lee C, et al. Ultrasonic speed microscopy for imaging of coronary artery. Ultrasonics 2016; 44: e51–e55.

Miura K, Katoh H. Structural and histochemical alterations in the aortic valves of elderly patients: a comparative study of aortic stenosis, aortic regurgitation, and normal valves. Biomed Res Int. 2016; 6125204.

Kobayashi K, Yoshida S, Saijo Y, et al. Acoustic impedance microscopy for biological tissue characterization. Ultrasonics 2014; 54: 1922–28.

Hatori K, Saijo Y, Hagiwara Y, et al. Acoustic diagnosis device for dentistry. Interface Oral Heal Sci. 2016; 181–201.

Gomez MR, Cerutti S, Sombre LL, et al. Determination of heavy metals for the quality control in argentinian herbal medicines by ETAAS and ICP-OES. Food Chem Toxicol. 2007; 45(6): 1060–64.

Junior AFS, Matos R, Andrade EM, et al. Multielement determination of macro and micro contents in medicinal plants and phytomedicines from brazil by ICP OES. J Braz Chem Soc. 2017; 28(2): 376–84.

Karıs D, Tarhan D, Boyacıoglu K, et al. The comparison of zinc, copper and iron levels in serum, aorta and left internal mammarian artery tissues in coronary by-pass graft surgery patients. J Trace Elem Medicine Biol. 2019; 51: 86–90.

Bilen B, Gokbulut B, Kafa U, et al. Scanning acoustic microscopy and time-resolved fluorescence spectroscopy for characterization of atherosclerotic plaques. Sci Reports 2018; 8: 14378 .

Kavlock R, Barr D, Boekelhaıde K, et al. NTP-CERHR expert panel update on the reproductive and developmental toxicity of di(2-ethylhexyl) phthalate. Reproductive Toxicol. 2006; 22: 291–399.

Kavlock R, Boekelhaide K, Chapin R, et al. Ntp center for evaluation risks to human reproduction: phthalates expert panel report on the reproductive and developmental toxicity of di-n-butyl phthalate. Reproductive Toxicol. 2002; 16: 489–527.

Prasanth GK, Divya LM, Sadasivan CN. Effects of mono and di(nbutyl) phthalate on superoxide dismutase. Toxicology, 2009; 262(1): 38–42.

Tetz LM, Cheng AA, Korte CS, et al. Mono-2-ethylhexyl phthalate induces oxidative stress responses in human placental cells in vitro. Toxicol Appl Pharmacol. 2013; 268(1): 47–54.

Sedha S, Kumar S, Shukla S. Role of oxidative stress in male reproductive dysfunctions with reference to phthalate compounds. Urol Journal. 2015; 12(5): 2304–16.

Sobarzo CM, Nde MR, Livia L. Et al. Mono-(2-ethylhexyl) phthalate (MEHP) affects intercellular junctions of sertoli cell: A potential role of oxidative stress. Reproductive Toxicol. 2015; 58: 203–12.

Cho YJ, Park SB, Han MN. Di-(2-ethylhexyl)-phthalate induces oxidative stress in human endometrial stromal cells in vitro. Mol Cell Endocrinol. 2015; 407: 9–17.

Asghari MH, Saeidnia S, Abdollahi M. A review on the biochemical and molecular mechanisms of phthalate-induced toxicity in various organs with a focus on the reproductive system. Int J Pharmacol. 2015; 11(2): 95–105.

Lingren A, Lindquıst NG, Lyden A., et al. A whole body autoradiographic study on the distribution of 14C-labelled di-(2- ethylhexyl)phthalate in mice. Toxicology 1982; 23: 149–158.

Garberg P, Hogberg J. Selenium metabolism in isolated hepatocytes: inhibition of incorporation in proteins by mono(2-ethylhexyl)phthalate, a metabolite of the peroxisome proliferator di(2-ethylhexyl)phthalate. Carcinogenesis 1991; 12(1): 7–12.

Erkekoglu P, Rachidi W, Yuzugullu OG, et al. Induction of ROS, p53, p21 in DEHP- and MEHPexposed LNCaP cells-protection by selenium compounds. Food Chem Toxicol. 2011; 49(7): 1565–71.

Aydemir D, Karabulut G, Franci GS, et al. Impact of the di(2-ethylhexyl) phthalate administration on trace element and mineral levels in relation of kidney and liver damage in rats. Biol Trace Elem Res. 2018; 186(2): 474–88.

Aydemir D, Karabulut G, Gok M, et al. Data the DEHP induced changes on the trace element and mineral levels in the brain and testis tissues of rats. Data Brief. 2019; 26: 104526.