The effect of different doeses of aspirin application on oxidative stress in ovarian tissue
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Abstract
Objective: Aspirin is a non-steroidal anti-inflammatory drug with antioxidative properties. It is recommended to use different doses and durations according to the characteristics of the patient and the type of disease. Therefore, in this study, we aimed to investigate the effect of using aspirin at different doses and for different durations on oxidative stress in ovarian tissue.
Material and Methods: Female Wistar albino rats were divided into five groups. Group 1: control group, no special treatment was applied to the rats in this group. Group 2: 1 mg/kg aspirin was administered orally to the rats in this group every day for 28 days. Group 3: 3 mg/kg aspirin was administered orally to rats in this group every three days. Ggroup 4: 5 mg/kg aspirin was administered orally to rats in this group every five days. Group 5: 7 mg/kg aspirin was administered orally to the rats in this group once a week. After fasting overnight following the last application, the rats were sacrificed, and their ovarian tissues were collected. Malondialdehyde, catalase, total thiol group, and AOPP levels were studied from ovarian tissue.
Results: Group4 and group5 ovarian tissue MDA levels were found to be significantly higher than the other groups (p<0.05). There was no significant difference between group1, group2 and group3 ovarian tissue MDA levels (p>0.05). Group1 (control group) ovarian tissue AOPP level was found to be significantly lower than all aspirin-administered groups (p<0.05). Group2 ovarian tissue AOPP level was found to be significantly lower than group3, group4 and group5 (p<0.05). TSG level was found to be significantly higher in group 5 when compared to other groups (p0<0.05). Group4 ovarian tissue TSG level was found to be significantly higher when compared to group1, group2 and group3 (p<0.05). Group3 and group4 ovarian tissue CAT activity was found to be significantly higher than group1, group2 and group5 (p<0.05). When group1, group2 and group5 ovarian tissue CAT activities were compared, no significant difference was found (p>0.05).
Conclusion: The application of aspirin at certain intervals rather than daily application may have more positive effects on the antioxidant system. especially taking aspirin at intervals of 3 or 5 days may be more effective
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Accepted 2021-08-11
Published 2021-08-16
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