Serum 25-Hydroxyvitamin D level in Restless Legs Syndrome: A controlled study

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Betül Çiftçi
Sacide Nur Saraçgil Coşar
Aslıhan Uzunkulaoğlu


Objective: We aimed to investigate 25-hydroxyvitamin D (25-OHD) level on a group of restless legs syndrome (RLS) patients.

Material and Methods: Twenty-one RLS patients and fourteen age and gender healthy controls (non RLS) with similar age and gender were included in the study. All patients underwent a physical examination for the neurologic and musculoskeletal system. The 25-OHD level was measured for all participants.  

Results: The 25-hydroxyvitamin D levels were higher in the RLS patient group (20.06±8.79 ng/ml) than in the healthy control group (14.75±4.67 ng/ml). In the healthy control group ‘’without the RLS syndrome’’, 25-hydroxyvitamin D levels were below the normal range (<20 ng/ml). However, there were no statistical differences in terms of vitamin D level between the RLS and Control groups (p>0.05).

Conclusion: In this study, due to the absence of RLS syndrome in the control group despite low 25-hydroxyvitamin D levels (<20 ng/ml), a relationship could not be established between 25-hydroxyvitamin D levels and RLS syndrome. The cause of RLS syndrome does not seem to depend on a single parameter such as 25-OHD level.


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How to Cite
Çiftçi, B., Saraçgil Coşar, S. N., & Uzunkulaoğlu, A. (2022). Serum 25-Hydroxyvitamin D level in Restless Legs Syndrome: A controlled study. Medical Science and Discovery, 9(1), 34–38.
Research Article
Received 2022-01-14
Accepted 2022-01-24
Published 2022-01-26


Allen RP, Walters AS, Montplaisir J, Hening W, Myers A, Bell TJ, et al. Restless legs syndrome prevalence and impact: REST general population study. Arch Intern Med. 2005;165(11):1286-1292. doi:10.1001/archinte.165.11.1286 DOI:

Coccagna G, Vetrugno R, Lombardi C, Provini F. Restless legs syndrome: an historical note. Sleep Med. 2004;5(3):279-283. doi:10.1016/j.sleep.2004.01.002 DOI:

Silber MH, Becker PM, Earley C, Garcia-Borreguero D, Ondo WG; Medical Advisory Board of the Willis-Ekbom Disease Foundation. Willis-Ekbom Disease Foundation revised consensus statement on the management of restless legs syndrome. Mayo Clin Proc. 2013;88(9):977-986. doi:10.1016/j.mayocp.2013.06.016 DOI:

Rana AQ, Khan F, Mosabbir A, Ondo W. Differentiating nocturnal leg cramps and restless legs syndrome. Expert Rev Neurother. 2014;14(7):813-818. doi:10.1586/14737175.2014.927734 DOI:

Nagandla K, De S. Restless legs syndrome: pathophysiology and modern management. Postgrad Med J. 2013;89(1053):402-410. doi:10.1136/postgradmedj-2012-131634. DOI:

Cherniack EP, Troen BR, Florez HJ, Roos BA, Levis S. Some new food for thought: the role of vitamin D in the mental health of older adults. Curr Psychiatry Rep. 2009;11(1):12-19. doi:10.1007/s11920-009-0003-3 DOI:

Wang JY, Wu JN, Cherng TL, Hoffer BJ, Chen HH, Borlongan CV, et al. Vitamin D(3) attenuates 6-hydroxydopamine-induced neurotoxicity in rats. Brain Res. 2001;904(1):67-75. doi:10.1016/s0006-8993(01)02450-7 DOI:; 2012

Allen RP, Picchietti DL, Garcia-Borreguero D, Ondo WG, Walters AS, Winkelman JW, et al. Restless legs syndrome/Willis-Ekbom disease diagnostic criteria: updated International Restless Legs Syndrome Study Group (IRLSSG) consensus criteria--history, rationale, description, and significance. Sleep Med. 2014;15(8):860-873. doi:10.1016/j.sleep.2014.03.025 DOI:

Romagnoli E, Pepe J, Piemonte S, Cipriani C, Minisola S. Management of endocrine disease: value and limitations of assessing vitamin D nutritional status and advised levels of vitamin D supplementation. Eur J Endocrinol. 2013;169(4):R59-R69. doi: 10.1530/EJE-13-0435. DOI:

Rye DB, Trotti LM. Restless legs syndrome and periodic leg movements of sleep. Neurol Clin. 2012;30(4):1137-1166. doi:10.1016/j.ncl.2012.08.004 DOI:

Rizzo V, Aricò I, Liotta G, Ricciardi L, Mastroeni C, Morgante F, et al. Impairment of sensory-motor integration in patients affected by RLS. J Neurol. 2010;257(12):1979-1985. doi:10.1007/s00415-010-5644-y DOI:

Patel N, Jankovic J, Hallett M. Sensory aspects of movement disorders. Lancet Neurol. 2014;13(1):100-112. doi:10.1016/S1474-4422(13)70213-8 DOI:

Ku J, Cho YW, Lee YS, Moon HJ, Chang H, Earley CJ, et al. Functional connectivity alternation of the thalamus in restless legs syndrome patients during the asymptomatic period: a resting-state connectivity study using functional magnetic resonance imaging. Sleep Med. 2014;15(3):289-294. doi:10.1016/j.sleep.2013.09.030 DOI:

Montplaisir J, Boucher S, Poirier G, Lavigne G, Lapierre O, Lespérance P. Clinical, polysomnographic, and genetic characteristics of restless legs syndrome: a study of 133 patients diagnosed with new standard criteria. Mov Disord. 1997;12(1):61-65. doi:10.1002/mds.870120111 DOI:

Earley CJ, Connor JR, Beard JL, Clardy SL, Allen RP. Ferritin levels in the cerebrospinal fluid and restless legs syndrome: effects of different clinical phenotypes. Sleep. 2005;28(9):1069-1075. doi:10.1093/sleep/28.9.1069 DOI:

Wrzosek M, Łukaszkiewicz J, Wrzosek M, Jakubczyk A, Matsumoto H, Piątkiewicz P. et al. Vitamin D and the central nervous system. Pharmacol Rep. 2013;65(2):271-278. doi:10.1016/s1734-1140(13)71003-x DOI:

Cui X, Pelekanos M, Liu PY, Burne TH, McGrath JJ, Eyles DW. The vitamin D receptor in dopamine neurons; its presence in human substantia nigra and its ontogenesis in rat midbrain. Neuroscience. 2013;236:77-87. doi:10.1016/j.neuroscience.2013.01.035 DOI:

Ibi M, Sawada H, Nakanishi M, Kume T, Katsuki H, Kaneko S, et al. Protective effects of 1 alpha,25-(OH)(2)D(3) against the neurotoxicity of glutamate and reactive oxygen species in mesencephalic culture. Neuropharmacology. 2001;40(6):761-771. doi:10.1016/s0028-3908(01)00009-0 DOI:

Oran M, Unsal C, Albayrak Y, Tulubas F, Oguz K, Avci O, et al. Possible association between vitamin D deficiency and restless legs syndrome. Neuropsychiatr Dis Treat. 2014;10:953-958. Published 2014 May 21. doi:10.2147/NDT.S63599 DOI:

Çakır T, Doğan G, Subaşı V, Filiz MB, Ülker N, Doğan ŞK, et al. An evaluation of sleep quality and the prevalence of restless leg syndrome in vitamin D deficiency. Acta Neurol Belg. 2015;115(4):623-627. doi:10.1007/s13760-015-0474-4 DOI:

Balaban H, Yıldız ÖK, Çil G, Şentürk İA, Erselcan T, Bolayır E, et al. Serum 25-hydroxyvitamin D levels in restless legs syndrome patients. Sleep Med. 2012;13(7):953-957. doi:10.1016/j.sleep.2012.04.009 DOI:

Stamp TC, Round JM. Seasonal changes in human plasma levels of 25-hydroxyvitamin D. Nature. 1974;247(5442):563-565. doi:10.1038/247563a0 DOI:

Lester E, Skinner RK, Wills MR. Seasonal variation in serum-25-hydroxyvitamin-D in the elderly in Britain. Lancet. 1977;1(8019):979-980. doi:10.1016/s0140-6736(77)92280-2 DOI:

Schramm S, Lahner H, Jöckel KH, Erbel R, Führer D, Moebus S; Heinz Nixdorf Recall Study Group. Impact of season and different vitamin D thresholds on prevalence of vitamin D deficiency in epidemiological cohorts-a note of caution. Endocrine. 2017;56(3):658-666. doi:10.1007/s12020-017-1292-7 DOI:

Cinar N, Harmanci A, Yildiz BO, Bayraktar M. Vitamin D status and seasonal changes in plasma concentrations of 25-hydroxyvitamin D in office workers in Ankara, Turkey. Eur J Intern Med. 2014;25(2):197-201. doi:10.1016/j.ejim.2013.11.004 DOI: