Examination the effects of chestnut and Manuka Honey for wound healing on mice experimental model
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Abstract
Objective: In this study, the aim is to examine the effects of Chestnut and Manuka honey on wound healing in order to investigate the effectiveness of cost-effective alternative to current approaches in terms of wound care and treatment.
Material and Methods: In this study, we used 30 healthy male Balbc mice weighing 18-24 g. We randomly divided the rats into three groups. A control group, a group treated with only Chestnut honey, a group treated with Manuka honey cream. After the wounds were formed in groups, the tissue samples were gathered on the seventh and fourteenth days. Then these samples were examined histologically and immunohistochemically.
Results: When the study results were evaluated, statistically significant differences were seen between histological and immune-histochemical findings in wound tissue preparations. On the seventh day, tissue samples showed re-epithelialization (P=0,002), granulation cell density (P=0,003) and angiogenesis (P= 0,003). In the fourteenth day tissue samples, we found epithelialization (P=0,001), granulation cell density (P=0,002) and angiogenesis (P=0,001). In the tissue samples in the seventh and fourteenth days between the groups, we found immino-histochemically, Ki-67 and EGF dyeing percentages as P= 0,004 and P=0,003 respectively.
Conclusion: We think chestnut honey may contribute to a shorter wound healing process.
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Accepted 2022-03-14
Published 2022-03-26
References
Drosou A, Falabella A, Kirsner R. Antiseptics on Wounds: An Area of Controversy. Wounds. 2003;15:149-66.
Vural F, Savci A. Yara Bakımında Yeni Uygulamalar New Practices in Wound Care. Turkiye Klinikleri Journal of Nursing Sciences. 2017;3:224-32.
Mendez-Eastman S. Wound dressing categories. Plastic Surgical Nursing. 2005;25(2):95-9.
Yaghoobi R, Kazerouni A, Kazerouni O. Evidence for Clinical Use of Honey in Wound Healing as an Anti-bacterial, Anti-inflammatory Anti-oxidant and Anti-viral Agent: A Review. Jundishapur journal of natural pharmaceutical products. 2013;8(3):100-4.
Iftikhar F, Arshad M, Rasheed F, Amraiz D, Anwar P, Gulfraz M. Effects of acacia honey on wound healing in various rat models. Phytotherapy research : PTR. 2010;24(4):583-6.
Kamaratos AV, Tzirogiannis KN, Iraklianou SA, Panoutsopoulos GI, Kanellos IE, Melidonis AI. Manuka honey-impregnated dressings in the treatment of neuropathic diabetic foot ulcers. International wound journal. 2014;11(3):259-63.
Mayer A, Slezak V, Takac P, Olejnik J, Majtan J. Treatment of non-healing leg ulcers with honeydew honey. Journal of tissue viability. 2014;23(3):94-7.
Stadler I, Zhang RY, Oskoui P, Whittaker MS, Lanzafame RJ. Development of a simple, noninvasive, clinically relevant model of pressure ulcers in the mouse. Journal of investigative surgery : the official journal of the Academy of Surgical Research. 2004;17(4):221-7.
Ulloa-Padilla JP, Ghassibi MP, Dubovy SR, Kerr DA. Clinicopathologic Correlation of Kaposi Sarcoma Involving the Ocular Adnexa: Immunophenotyping of Diagnostic and Therapeutic Targets. Ophthalmic plastic and reconstructive surgery. 2020;36(2):185-90.
Reid RR, Sull AC, Mogford JE, Roy N, Mustoe TA. A novel murine model of cyclical cutaneous ischemia-reperfusion injury. The Journal of surgical research. 2004;116(1):172-80.
Al-Mamary M, Al-Meeri A, Al-Habori M. Antioxidant activities and total phenolic of different types of honey. Nutrition Research - NUTR RES. 2002;22:1041-7.
Panchatcharam M, Miriyala S, Gayathri VS, Suguna L. Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species. Molecular and cellular biochemistry. 2006;290(1-2):87-96.
Akershoek JJ, Brouwer KM, Vlig M, Boekema B, Beelen RHJ, Middelkoop E, et al. Differential effects of Losartan and Atorvastatin in partial and full thickness burn wounds. PloS one. 2017;12(6):e0179350.
Enoch S, Leaper DJ. Basic science of wound healing. Surgery (Oxford). 2005;23(2):37-42.
Czubryt MP. Common threads in cardiac fibrosis, infarct scar formation, and wound healing. Fibrogenesis & Tissue Repair. 2012;5(1):19.
Mahdavian Delavary B, van der Veer WM, van Egmond M, Niessen FB, Beelen RH. Macrophages in skin injury and repair. Immunobiology. 2011;216(7):753-62.
Haryanto H, Urai T, Mukai K, Gontijo Filho PP, Suriadi S, Sugama J, et al. Effectiveness of indonesian honey on the acceleration of cutaneous wound healing: an experimental study in mice. Wounds. 2012;24(4):110-9.
Nisbet HO, Nisbet C, Yarim M, Guler A, Ozak A. Effects of three types of honey on cutaneous wound healing. Wounds: a Compendium of Clinical Research and Practice. 2010 Nov 1;22(11):275-83.
Profyris C, Tziotzios C, Do Vale I. Cutaneous scarring: Pathophysiology, molecular mechanisms, and scar reduction therapeutics Part I. The molecular basis of scar formation. Journal of the American Academy of Dermatology. 2012;66(1):1-10; quiz 1-2.
Werner S, Grose R. Regulation of wound healing by growth factors and cytokines. Physiological reviews. 2003;83(3):835-70.
Mukai K, Koike M, Nakamura S, Kawaguchi Y, Katagiri F, Nojiri S, et al. Evaluation of the effects of a combination of Japanese honey and hydrocolloid dressing on cutaneous wound healing in male mice. Evidence-based complementary and alternative medicine : eCAM. 2015;2015:910605.
Park J-S, An S-J, Jeong S-I, Gwon H-J, Lim Y-M, Nho Y-C. Chestnut Honey Impregnated Carboxymethyl Cellulose Hydrogel for Diabetic Ulcer Healing. Polymers (Basel). 2017;9(7):248.