Detection of Viral Respiratory Factors via Multiplex PCR in Newborn & Pediatric Patients and Their Distribution According to Seasons
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Abstract
Objective: Respiratory viruses are a global public health problem, and viruses cause up to 80% of respiratory infections. This study aimed to elucidate the viral respiratory tract factors and the frequency of coinfections in the newborn and pediatric age groups determined by the molecular respiratory tract panel (MRTP) kit.
Materials & Method: The results of the respiratory tract panel test with the molecular multiplex method were applied to 1486 newborn and pediatric patients between 01.10.2020 and 30.04.2022 to determine the viral respiratory tract factors were analyzed retrospectively. The Multiplex RT – PCR test confirmed results were recorded from the hospital database under the supervision of a microbiologist, negative and positive controls were evaluated, and test was validated.
Results: Clinical virology laboratory test results were scanned and at least one respiratory tract virus was detected in nasopharyngeal swabs of 499 (33.6%) patients. A total of 634 viruses were detected in 499 NS-positive samples. The most commonly detected viral pathogens were parainfluenza – 3 (36.9%, n=184), respiratory syncytial virus (22.8%, n=114), human rhinovirus (19.2%, n=96), SARS-CoV-2 (12.6%, n=63), and human bocavirus (10.8%, n=54) respectively.
Conclusion: In this research, we tried to elaborate the accuracy of molecular multiplex method and the respiratory tract panel test to determine the respiratory factors in newborn and pediatric age group patients. The logic behind this lies beneath the fact that diagnosing with a kit that can detect both single and multiple factors causing coinfection can be performed simultaneously.
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Accepted 2022-10-21
Published 2022-10-24
References
Núñez-Samudio, V., & Landires, I. (2021). Epidemiology of viral respiratory infections in a pediatric reference hospital in Central Panama. BMC infectious diseases, 21(1), 43. https://doi.org/10.1186/s12879-020-05720-1 DOI: https://doi.org/10.1186/s12879-020-05720-1
Tan, K., Yung, C. F., Maiwald, M., Saffari, S. E., Thoon, K. C., & Chong, C. Y. (2021). Respiratory viral infections in hospitalised paediatric patients in the tropics. Journal of paediatrics and child health, 57(4), 559–565. https://doi.org/10.1111/jpc.15267 DOI: https://doi.org/10.1111/jpc.15267
Ostrow, O., Savlov, D., Richardson, S. E., & Friedman, J. N. (2022). Reducing Unnecessary Respiratory Viral Testing to Promote High-Value Care. Pediatrics, 149(2), e2020042366. https://doi.org/10.1542/peds.2020-042366 DOI: https://doi.org/10.1542/peds.2020-042366
Shi T, McAllister DA, O’Brien KL, Simoes EAF, Madhi SA, Gessner BD, et al. Global, regional, and national disease burden estimates of acute lower respiratory infections due to respiratory syncytial virus in young children in 2015: a systematic review and modelling study. Lancet. 2017;390(10098):946–958. DOI: https://doi.org/10.1016/S0140-6736(17)30938-8
Shen KL, Namazova-Baranova L, Yang YH, Wong GWK, Rosenwasser LJ, Rodewald LE, et al.; Global Pediatric Pulmonology Alliance (GPPA) Expert Panel on Infectious Diseases & COVID-19. Global Pediatric Pulmonology Alliance recommendation to strengthen prevention of pediatric seasonal influenza under COVID-19 pandemic. World J Pediatr. 2020 Oct;16(5):433-437. doi: 10.1007/s12519-020-00389-7. Epub 2020 Sep 13. DOI: https://doi.org/10.1007/s12519-020-00389-7
Kehl SC, Kumar S. Utilization of nucleic acid amplification assays for the detection of respiratory viruses. Clin Lab Med, 2009; 29(4):661-671. DOI: https://doi.org/10.1016/j.cll.2009.07.008
Sadeghi CD, Aebi C, Gorgievski-Hrisoho M, Mühlemann K, Barbani MT. Twelve years' detection of respiratory viruses by immunofluorescence in hospitalised children: impact of the introduction of a new respiratory picornavirus assay. BMC Infect Dis, 2011 7; 11:41. DOI: https://doi.org/10.1186/1471-2334-11-41
Carr J, Williams DG, Hatden RT. Molecular detection of multiple respiratory viruses. Wayne W. Grody WW, Nakamura RM, Kiechle FL, Strom C. Molecular Diagnostics: Techniques and Applications for the Clinical Laboratory. 1st ed, USA: Elsevier Inc., 2010; 289-300. DOI: https://doi.org/10.1016/B978-0-12-369428-7.00024-0
Yan Y, Zhang S, Tang YW. Molecular assays for the detection and characterization of respiratory viruses. Semin Respir Crit Care Med, 2011; 32(4):512-526. DOI: https://doi.org/10.1055/s-0031-1283288
Caliendo AM. Multiplex PCR and emerging technologies for the detection of respiratory pathogens. Clin Infect Dis, 2011; 52 Suppl 4:S326-330. DOI: https://doi.org/10.1093/cid/cir047
Gonzalez-Carrasco E, Calvo C, García-Garcia ML, Beato M, Muñoz-Archidona C, Pozo F, et al. Viral respiratory tract infections in the neonatal intensive care unit. An Pediatr (Engl Ed) 2015;82:242-246. DOI: https://doi.org/10.1016/j.anpede.2015.03.007
Epalza C, Hallin M, Busson L, Debulpaep S, De Backer P, Vandenberg O, et al. Role of Viral Molecular Panels in Diagnosing the Etiology of Fever in Infants Younger Than 3 Months. Clin Pediatr (Phila) 2020;59:45-52. DOI: https://doi.org/10.1177/0009922819884582
Kidszun A, Klein L, Winter J, Schmeh I, Gröndahl B, Gehring S, et al. Viral Infections in Neonates with Suspected Late-Onset Bacterial Sepsis-A Prospective Cohort Study. Am J Perinatol 2017;34:1-7. DOI: https://doi.org/10.1055/s-0036-1584150
Garcia CG, Bhore R, Soriano-Fallas A, Trost M, Chason R, Ramilo O, et al. Risk factors in children hospitalized with RSV bronchiolitis versus non-RSV bronchiolitis. Pediatrics 2010;126(6):e1453-60. DOI: https://doi.org/10.1542/peds.2010-0507
Stockman LJ, Curns AT, Anderson LJ, Fischer-Langley G. Respiratory syncytial virus-associated hospitalizations among infants and young children in the United States, 1997-2006. Pediatr Infect Dis J 2012;31:5-9. DOI: https://doi.org/10.1097/INF.0b013e31822e68e6
Cho HJ, Shim SY, Son DW, Sun YH, Tchah H, Jeon IS. Respiratory viruses in neonates hospitalized with acute lower respiratory tract infections. Pediatr Int 2013;55:49-53. DOI: https://doi.org/10.1111/j.1442-200X.2012.03727.x
Bukhari EE, Elhazmi MM. Viral agents causing acute lower respiratory tract infections in hospitalized children at a tertiary care center in Saudi Arabia. Saudi Med J 2013;34:1151-5.
Homaira N, Sheils J, Stelzer-Braid S, Lui K, Oie JL, Snelling T, et al. Respiratory syncytial virus is present in the neonatal intensive care unit. J Med Virol 2016;88:196-201. DOI: https://doi.org/10.1002/jmv.24325
Chien-Yu Lin, David Hwang, Nan-Chang Chiu, Li-Chuan Weng, Hsin-Fu Liu, Jung-Jung Mu, et al., Increased Detection of Viruses in Children with Respiratory Tract Infection Using PCR, Int. J. Environ. Res. Public Health 2020, 17, 564 DOI: https://doi.org/10.3390/ijerph17020564
Jansen RR, Schinkel J, Koekkoek S, Pajkrt D, Beld M, de Jong MD, Molenkamp R. Development and evaluation of a four-tube real time multiplex PCR assay covering fourteen respiratory viruses, and comparison to its corresponding single target counterparts. J Clin Virol, 2011; 51(3):179-185. DOI: https://doi.org/10.1016/j.jcv.2011.04.010
Ozcan C, Toyran M, Civelek E, Erkoçoğlu M, Altaş AB, Albayrak N, Korukluoğlu G, Kocabaş CN. Evaluation of respiratory viral pathogens in acute asthma exacerbations during childhood. J Asthma, 2011; 48(9):888-893. DOI: https://doi.org/10.3109/02770903.2011.606579
Beka H, Kilic A, Unuvar E, Onel M, Oguz F, Sidal M, Aslan S, Bozkaya E, Badur S, Agacfidan A. Frequency of common viruses in etiology of acute respiratory tract infections. Indian J Pediatr, 2013; 80(2):91-96. DOI: https://doi.org/10.1007/s12098-012-0880-z