Investigation of the changes in some elements that play an active role in MS disease

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Soner Kılıç


Objective: In this study, we examined the role of several metals which extracted from the hair of patients who affected by multiple sclerosis (MS). The ICP-MS measurements were performed using an Agilent 7500 series. With this procedure, all elements can be simultaneously quantified.

Material and Methods: All studies were carried out at Kayseri City Hospital between August 9, 2021, and October 11, 2021. We measured five elements from hair in association with the clinical course in 40 male and female MS subjects, as well as 40 healthy volunteers, using 1 cm hair sections. Using a novel microwave digestion method coupled with ICP-MS, we were able to analyze large numbers of samples per day consisting of 0.5 g of hair in 5 mL of nitric acid. Following digestion, the aliquots were examined to determine the quantities of Hg, Pb, Cu, Cd, and Zn.

Results: The quantities recovered for all elements were in an acceptable certified value range of (97.60–99.49%). Cu, Zn, and Hg levels examined were higher in the patient group, and Cd and Pb were higher in the control group. In our patients, a positive correlation was found between age, Hg, Cd, Pb values, and the disability score of multiple sclerosis [EDSS], while there was a negative correlation between Zn and Cu values and EDSS. Additionally, female MS patients showed a higher average hair content than males for the elements studied. Both Cd and Hg levels in patients were increased compared to the controls. Lead content in hair is steadily increasing in individuals of two different age groups. Lead content in hair showed an increased trend with age in the 21-35 year and 36-60 age groups.

Conclusion: Our study shows some important changes and relationships with Pb. Also, it has been determined that the Pb element increases with age, and the Cd and Hg levels were noticeably different in the patient and healthy groups, and Cu and Zn values change in parallel. The study can be a guide for diagnosis and treatment of the MS.


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How to Cite
Kılıç, S. (2023). Investigation of the changes in some elements that play an active role in MS disease. Medical Science and Discovery, 10(3), 154–159.
Research Article
Received 2023-02-03
Accepted 2023-03-06
Published 2023-03-14


Kintz P (2017a) Evidence of 2 populations of mephedrone abusers by hair testing. Application to 4 forensic expertises. Curr Neuropharmacol 15:658–662.

Montesano C, Vannutelli G, Massa M, Simeoni MC, Gregori A, Ripani L, Compagnone D, Curini R, Sergi M (2017) Multi-class analysis of new psychoactive substances and metabolites in hair by pressurized liquid extraction coupled to HPLC-HRMS. Drug Test Anal 9:798–807.

M. R. Werbach, Lab methods for nutritional evaluation, in Nutritional Influences on Illness, 2nd ed., Third Line Press, Tarzana, CA, pp. 642-654 (1993).

S. C. Foo, N. Y. Khoo, A. Heng, J. H. Chua, S. E. Chia, C. N. Ong, et al., Metals in hair as biological indices for exposure, Int. Arch. Occup. Environ. Health 65, $83-$86 (1993).

M. A. Bosque, J. L. Domingo, J. M. Llobet, and J. Corbella, Cadmium in hair of school children living in Tarragona Province, Spain, Biol. Trace Element Res. 28, 147-155 (1991).

A. C. K. Man, Y. Zheng, and P. Mak, Trace elements in scalp hair of professional drivers and university teachers in Hong Kong, Biol. Trace Element Res. 53, 241-247 (1996).

B. Huang, S. Lin, S. Chen, G. Zhou, F. Yin, Z. Lou, et al., Hair chromium levels in patients with vascular diseases, Biol. Trace Element Res. 29, 133-137 (1991).

O. Donma, S. Gunbey, M. A. Tas, and M. M. Donma, Zinc, copper, and magnesium concentrations in hair, of children from Southeastern Turkey, Biol. Trace Element Res. 24, 39-47 (1990).

A. O. Cavdar, M. Bahceci, N. Akar, F. N. Dincer, and J. Erten, Biol. Trace Element Res. 30, 81-85 (1991).

A. Aharoni, B. Tesler, Y. Paltieli, J. Tal, Z. Dori, and M. Sharf, Hair chromium content of women with gestational diabetes compared with nondiabetic pregnant woman, Am. J. Clin. Nutr. 55, 104-107 (1992).

T. Suzuki, T. Hongo, J. Yoshinaga, H. Imai, M. Nakazawa, N. Matsuo, and H. Akagi, The hair-organ relationship in mercury concentration in contemporary Japanese, Arch. Environ. Health 48, 221-229 (1993).

P. K. Chattopadhyay, H. C. Joshi, and K. R. Samaddar, Hair cadmium level of smoker and non-smoker human volunteers in and around Calcutta City, Bull. Environ. Contam. Toxicol. 45, 177-180 (1990).

S. A. Katz and A. Chatt, The significance of hair analysis, in Hair Analysis: Applications in the Biomedical and Environmental Sciences, VCH Publishers, NY, pp. 105-109 (1988).

F.J. Roberts, L. Ebdon, S.J. HillA preliminary clinical investifation into the potential of

blood lead levels to measure bone resorption in patients with skeletal metastases using ICP- MS, J. Trace Elem. Med. Biol., 14 (2000), pp. 108-115.

R.H. Gwiazda, D.R. SmithLead isotopes as a supplementary tool in the routine evaluation of household lead hazards Environ. Health Perspect., 108 (2000), pp. 1091-1097.

K. Inagaki, H. HaraguchiDetermination of rare earth elements in human blood serum by inductively coupled plasma mass spectrometry after chelating resin preconcentration, Analyst, 125 (2000), pp. 191-196.

R. Forrer, K. Gautschi, H. LutzSimultaneous measurement of the trace elements Al, As, B, Be, Cd, Co, Cu, Fe, Li, Mn, Mo, Ni, Rb, Se, Sr and Zn in human serum and their reference ranges by ICP-MS, Biol. Trace Elem. Res., 80 (2001), pp. 77-93.

M. Krachler, K.J. IrgolicThe potential of inductively coupled plasma mass spectrometry (ICP-MS) for the simultaneous determination of trace elements in whole blood, plasma and serum, J. Trace Elem. Med. Biol., 13 (1999), pp. 157-169.

B. Bouvier-Capely, J. Ritt, N. Baglan, C. CossonnetPotentialities of mass spectrometry (ICP-MS) for actinides determination in urine, Appl. Radiat. Isot., 60 (2004), pp. 629-633.

C. Benes, K. Jakubec, J. Smid, V. SpevachovaDetermination of thirty-two elements in human autopsy tissue, Biol . Trace Elem. Res., 75 (2000), pp. 195-203.

Ebers GC, Sadovnick AD. The role of genetic factors in multiple sclerosis susceptibility. J Neuroimmunol. 1994; 54:1–17. PMID: 7929798.

Sadovnick AD, Ebers GC, Dyment DA Risch NJ, Canadian CSG. Evidence for the genetic basis of multiple sclerosis. Lancet 1996; 347:1728–1730. PMID: 8656905.

Ryan DE, Holzbecher J, Stuart DG. Trace Elements in Scalp-Hair of Persons with Multiple Sclerosis and of Normal Individuals. Clin Chem. 1978; 24: 11.

Schulten HR, Monkhouse PB, Achenbach C, Ziskoven R. Determination of rubidium in human serum. Experientia. 1983; 15; 39(7): 736–8. PMID: 6345189

Sayre LM, Perry G, Atwood CS, Smith MA. The role of metals in neurodegenerative

diseases. Cell Mol Biol. 2000; 46: 731–41. PMID: 10875436

Bomboi G, Marchione F, Sepe-Monti M, De Carolis A, Bianchi V, Medda E, et al. Correlation between metal ions and clinical findings in subjects affected by Alzheimer’s disease. Ann Ist Super Sanità 2005; 41(2): 205–212.

Forte G, Alimonti A, Violante N, Di Gregorio M, Senofonte O, Petrucci F, et al. Calcium, copper, iron, magnesium, silicon and zinc content of hair in Parkinson’s disease. J Trace Elem Med Bio. 2005; 19: 195–201. PMID: 16325536

Exley C, House ER. Aluminium in the human brain. Monatsh Chem. 2010; 142: 357–363.

Vinceti M, Solovyev N, Mandrioli J, Crespi CM, Bonvicini F, Arcolin E, et al. Cerebrospinal fluid of newly diagnosed amyotrophic lateral sclerosis patients exhibits abnormal levels of selenium species including elevated selenite. Neurotox. 2013; 38: 25–32.

Nicoletti A, Patti F, Lo Fermo S, Messina S, Bruno E, Quattrocchi G, et al. Increasing frequency of multiple sclerosis in Catania, Sicily: a 30-year survey. Mult Scler. 2011; 17:273– 280. doi: 10.1177/ 1352458510386995 PMID: 21071466

Valera P, Zavattari P, Albanese S, Cicchella D, Dinelli E, Lima A, et al. A correlation study between multiple sclerosis and type 1 diabetes incidences and geochemical data in Europe. Environ Geoch Hlth. 2014; 36: 79–98.

Compston A (2016) The story of multiple sclerosis, McAlpine’s Multiple Sclerosis. p. 3- 68. 2. Stankiewicz J, Panter SS, Neema M, Arora A, Batt CE, et al. (2007) Iron in chronic brain disorders: imaging and neurotherapeutic implications. Neurotherapeutics 4(3): 371-386.

Y, Kawabata T, Kaneko Y, Kudoh H, et al. (2012) Influence of essential trace minerals and micronutrient insufficiencies on harmful metal overload in a Mongolian patient with multiple sclerosis. Curr Aging sci 5(2): 112-125.

Riise T, Moen BE, Kyvik KR (2002) Organic solvents and the risk of multiple sclerosis. Epidemiology 6: 718–720.

Grønning M, Albrektsen G, Kva˚le G, Moen B, Aarli JA, et al. (1993) Organic solvents and multiple sclerosis: a case-control study. Acta Neurol Scand 88: 247– 250. 31. Schiffer RB, Mcdermott MP, Copley C (2001) A Multiple Sclerosis Cluster Associated with a Small, North-Central Illinois Community. Arch Environ Health 56: 389–395.

Purdey M (2004) Chronic barium intoxication disrupts sulphated proteoglycan synthesis: a hypothesis for the origins of multiple sclerosis. Med Hypotheses 62: 746–754.

Johnson S (2000) The possible role of gradual accumulation of copper, cadmium, lead and iron and gradual depletion of zinc, magnesium, selenium, vitamins B2, B6, D and E and essential fatty acids in multiple sclerosis. Med Hypotheses 55: 239–241.

Kapaki E, Segditsa J, Papageorgiou C (1989) Zinc, copper and magnesium concentration in serum and CSF of patients with neurological disorders. Acta Neurol Scand 79: 373–378.

Larsen C, White LR, Aasly J, Sjøbakk TE, Flaten TP, et al. (2003) Manganese, copper, and zinc in cerebrospinal fluid from patients with multiple sclerosis. Biol Trace Elem Res 93:

Fathi F and Ghaffari-Cherati, Metals in Multiple Sclerosis: Improvement or Progression, Review Article,Volume 2 Issue 2 - May 2017,Global journal of nano medicine

Dongarrà G, Varrica D, Tamburo E, D’Andrea D. Trace elements in scalp hair of children living in differing environmental contexts in Sicily (Italy). Environ Toxicol Pharmacol. 2012; 34: 160–9. doi: 10.1016/j.etap.2012.03.005 PMID: 22522426

Falchuk KH. The Molecular Basis for the Role of Zinc in Developmental Biology, Mol. Cell Biochem.1998; 188: 41–48. PMID: 9823009

G. ZHUNG, Y. CHENG, M. TAN, Y. WANG, M. ZHI, J. Radioanal. Nucl. Chem., 149 (1991) 305.

Nicoletti A, Bruno E, Nania M, Cicero E, Messina S, Chisari C, et al. Multiple Sclerosis in the Mount Etna Region: Possible Role of Volcanogenic Trace Elements. PLOS ONE 2014; 8 Issue 12: 1–6.

M. Etemadifar et al. Soil heavy metals are associated with the distribution of multiple sclerosis in Isfahan,IranActa Neurol Scand 2016;134:292-299