@article{Sakin_Alan_Anğın_Basak_Alan_2019, title={An investigation of the protective effects of Dehydroepiandrosterone (DHEA) in chemotherapatic Cyclophosphamide (CP) induced ovarian damage on rats }, volume={6}, url={https://medscidiscovery.com/index.php/msd/article/view/333}, DOI={10.36472/msd.v6i12.333}, abstractNote={<p><strong>Objective</strong>: We aimed to investigate whether cyclophosphamide-induced damage to rat ovary can be prevented by DHEA.</p> <p><strong>Material and Method:</strong> Group 1 (the control Group): no treatment was administered. Intact ovarian tissue was removed and blood samples were taken for anti-mullerian hormone (AMH) test. Group 2 (the Cyclophosphamide Group): Rats received Cyclophosphamide intraperitoneally at a single dose of 150 mg / kg. Group 3 (the Cyclophosphamide + DHEA Group): Rats received Cyclophosphamide intraperitoneally at a single dose of 150 mg / kg at baseline and DHEA subcutaneously for 10 days at a dose of 60 mg / kg daily. Rats in groups 2 and 3 were sacrificed at the end of 10 days, ovarian tissues were removed and blood samples were taken for AMH test.</p> <p><strong>Results:</strong> While normal ovarian tissue damage scores were zero, cyclophosphamide showed significant damage and histopathological changes in all rats. Cyclophosphamide group had higher vascular congestion (p=0.004) and total damage scores (p=0.010) than normal ovarian group. Cyclophosphamide + DHEA group had higher edema (p<0.001), vascular congestion (p<0.001) and total damage scores (p<0.001). Cyclophosphamide group had a decrease in primordial (p = 0.001), primary (p = 0.043) and preantral follicles(p = 0.006). Cyclophosphamide + DHEA group showed a decrease in primordial (p = 0.001) and antral follicles(p = 0.018). AMH levels did not decrease in both groups.</p> <p><strong>Conclusions:</strong> It was found that the use of DHEA to prevent Cyclophosphamide-induced ovarian damage in rats did not produce significant changes in antral follicle counts, ovarian volume, and AMH levels, which were important for clinical practice.</p>}, number={12}, journal={Medical Science and Discovery}, author={Sakin, Önder and Alan, Yasemin and Anğın, Ali Doğukan and Basak, Kahyan and Alan, Murat}, year={2019}, month={Dec.}, pages={340–346} }