TY - JOUR AU - Şahin, Fatih AU - Özözen Şahin, Elif AU - Toptan, Hande AU - Köroğlu, Mehmet AU - Erdem, Ali Fuat AU - Yaylacı, Selçuk AU - karabay, Oğuz PY - 2021/12/23 Y2 - 2024/03/29 TI - Evaluation of using distal part of endotracheal tube samples for SARS-COV-2 diagnosis by RT-PCR JF - Medical Science and Discovery JA - Med Sci Discov VL - 8 IS - 12 SE - Research Article DO - 10.36472/msd.v8i12.640 UR - https://medscidiscovery.com/index.php/msd/article/view/640 SP - 716-720 AB - <p><strong>Objective:</strong> The reverse transcription-polymerase chain reaction (RT-PCR) analyses method is the most important diagnostic method in the diagnosis of SARS-CoV-2 virus infection. In this research, we  aimed to investigate the positivity of SARS-CoV-2 by RT-PCR from distal part of the endotracheal tube (DPET) samples, which have not been investigated in any study yet.</p><p><strong>Materials and Methods:</strong> A total of 48 patients with a diagnosis of COVID-19 hospitalized in the intensive care unit receiving mechanical ventilation and whose conditions resulted in death or extubation were included in the study. The distal 6 cm part of the orotracheal intubation tube was removed from the patient (including the cuff). DPET samples were mixed with viral transport medium and vortexed; then, it was centrifuged at 4500g for 4 minutes. RNA isolation was performed by taking 400 µl from the supernatant and then SARS-CoV-2 RT-PCR was studied.</p><p><strong>Results:</strong> In 15 patients (31.25 %) the swab samples were PCR positive, 42 patients (87.5 %) had positive computed tomography finding and 48 patients (100 %) had positive clinical findings. Among the patients whose oropharynx (OP)/nasopharynx (NP) combined swab sample was positive for RT-PCR, the rate of RT-PCR positivity detected in DPET samples was 26.7%. While OP/NP combined swab sample was negative, DPET RT-PCR positivity rate was found to be 9.09%.</p><p><strong>Conclusions:</strong> Patients with positive DPET RT-PCR are detected when the swab is negative. These findings suggest that DPET can be used as a good lower respiratory sample without the risk of particle spread and transmission to healthcare personnel.</p> ER -