Investigation of Vascular Endothelial Growth Factor and Endostatin Levels in Some Rat Tissues in Response to Cold Stress and Diet Angiogenic and antiantiogenic Factors in Obese Rats

Article Sidebar

Full Text (PDF)
Published: Mar 3, 2024
DOI: https://doi.org/10.36472/msd.v11i3.1117
Keywords:
VEGF Endostatin Obesity Propolis

Main Article Content

Filiz Coban
Inönü University, Institute of Science and Technology, Malatya, TR
https://orcid.org/0000-0002-8471-6983
Muhittin Yurekli
Inönü University, Faculty of Arts and Sciences, Malatya, TR
https://orcid.org/0000-0002-5830-8564

Abstract

Objective: Obesity, the disease of our age, is a condition that occurs when there is an excess of fat tissue in the body. It is not merely a concern about weight gain, but rather a medical issue that elevates the risk of various diseases including heart disease, diabetes, high blood pressure, and certain cancers. This study aimed to explore the impact of a high-fat diet under normal conditions and cold stress, as well as the influence of propolis as a dietary supplement, on vascular endothelial growth factor (VEGF) and endostatin levels in rats fed with propolis. 


Material and Methods: Thirty-six 3-month-old female Wistar rats (6 rats in each group) sourced from Inonu University Experimental Animal Production and Research Center were utilized for the study. Propolis was administered by gavage, dissolved in water, at a dosage of 2 mL per day for two weeks.


Results: The group exhibiting at least a 20% increase in weight due to high-fat diet consumption was categorized as the obese group. Tissues including heart, liver, lung, brown adipose, and white adipose tissues were procured from the obese, propolis-treated, and control groups. Endostatin and vascular endothelial growth factor levels were assessed in the tissues using the ELISA method. The study revealed an elevation in VEGF levels in brown adipose tissue in both cold stress and propolis treatment groups, accompanied by a reduction in white adipose tissue compared to the control group. Additionally, VEGF levels displayed a general increase in lung, liver, and heart tissues. Conversely, endostatin levels, an antiangiogenic factor, decreased in brown adipose tissue while increasing in white adipose tissue. In liver, lung, and heart tissues, endostatin levels exhibited a general decrease.


Conclusion: The findings suggest that both cold stress and propolis treatment influence VEGF and endostatin levels in various rat tissues, indicating potential implications for obesity-related conditions and angiogenesis regulation.

Downloads

Download data is not yet available.

Article Details

How to Cite
Coban, F., & Yurekli, M. (2024). Investigation of Vascular Endothelial Growth Factor and Endostatin Levels in Some Rat Tissues in Response to Cold Stress and Diet: Angiogenic and antiantiogenic Factors in Obese Rats . Medical Science and Discovery, 11(3), 73–80. https://doi.org/10.36472/msd.v11i3.1117
Issue
Vol. 11 No. 3 (2024): Medical Science and Discovery
Section
Research Article
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

https://creativecommons.org/licenses/by-nc/4.0/

Received 2024-01-10
Accepted 2024-02-29
Published 2024-03-03

References

Hiroshimaa Y, Yamamoto T, Watanabe M, Babad Y, Shinoharaa Y. Effects of cold exposure on metabolites in brown adipose tissue of rats. Mol Genet Metab Rep. 2018;15:36-42.

Couillard C, Maurie`ge P, Imbeault P, Prud'homme D, Nadeau A, Tremblay A, Bouchard C, Després JP. Hyperleptinemia is more closely associated with adipose cell hypertrophy than with adipose tissue hyperplasia. Int J Obes Relat Metab Disord. 2000;24(6):782-788.

Bouloumie´ A, Lolme`de K, Sengene`s C, Galitzky J, Lafontan M. Angiogenesis in adipose tissue. Ann Endocrinol (Paris). 2002;63:91-95.

Silverman KJ, Lund DP, Zetter BR, Lainey LL, Shahood JA, Freiman DG, Folkman JB. Angiogenic activity of adipose tissue. Biochem Biophys Res Commun. 1988;153:347-352.

Rupnick MA, Panigrahy D, Zhang CY, Dallabrida SM, Lowell BB, Langer R, Folkman MJ. Adipose tissue mass can be regulated through the vasculature. Proc Natl Acad Sci USA. 2002;99(16):10730-10735.

Brakenhielm E, Cao R, Gao B, Angelin B, Cannon B, Parini P, Cao Y. Angiogenesis inhibitor, TNP-470, prevents diet-induced and genetic obesity in mice. Circ Res. 2004;94(12):1579-1588.

Neels JG, Thinnes T, Loskutoff DJ. Angiogenesis in an in vivo model of adipose tissue development. FASEB J. 2004;18(9):983-985.

Folkman J. Clinical applications of research on angiogenesis. N Engl J Med. 1995;333(26):1757-1763.

Klagsbrun M, Moses MA. Molecular angiogenesis. Chem Biol. 1999;6(8):217-224.

Folkman J. Angiogenesis in cancer, vascular, rheumatoid and other disease. Nat Med. 1995;1(1):27-31.

Colpaert CG, Vermeulen PB, Benoy I, Soubry A, Van Roy F, van Beest P, Goovaerts G, Dirix LY, Van Dam P, Fox SB, Harris AL, Van Marck EA. Inflammatory breast cancer shows angiogenesis with high endothelial proliferation rate and strong E-cadherin expression. Br J Cancer. 2003;88(5):718-725.

Yoo SY, Kwon SM. Angiogenesis and its therapeutic opportunities. Mediators Inflamm. 2013;1-11.

Pepper MS. Role of the matrix metalloproteinase and plasminogen activator-plasmin systems in angiogenesis. Arterioscler Thromb Vasc Biol. 2001;21(7):1104-1117.

Mignatti P, Rifkin DB. Plasminogen activators and matrix metalloproteinases in angiogenesis. Enzyme Protein. 1996;49(1-3):117-137.

Rehman J, Traktuev D, Li J, Merfeld-Clauss S, Temm-Grove CJ, Bovenkerk JE, March KL. Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation. 2004;109(10):1292-1298.

Cao Y. Emerging mechanisms of tumor lymphangiogenesis and lymphatic metastasis. Nat Rev Cancer. 2005;5(9):735-743.

Li J, Yu X, Pan XA, Unger RH. Gene expression profile of rat adipose tissue at the onset of high-fat-diet obesity. Am J Physiol Endocrinol Metab. 2002;282(6):E1334-E1341.

Baillargeon J, Rose DP. Obesity, adipokines, and prostate cancer. Int J Oncol. 2006;28(3):737-745.

Voros G, Maquoi E, Demeulemeester D, Clerx N, Collen D, Lijnen HR. Modulation of angiogenesis during adipose tissue development in murine models of obesity. Endocrinology. 2005;146(10):4545-4554.

Dobson DE, Kambe A, Block E, Dion T, Lu H, Castellot Jr JJ, Spiegelman BM. 1-Butyryl-glycerol: a novel angiogenesis factor secreted by differentiating adipocytes. Cell. 1990;61(2):223-230.

Wilkison WO, Choy L, Spiegelman BM. Biosynthetic regulation of monobutyrin, an adipocyte-secreted lipid with angiogenic activity. J Biol Chem. 1991;266(25):16886-16891.

Cao Y. Angiogenesis modulates adipogenesis and obesity. J Clin Invest. 2007;117(9):2362-2368.

Zhang QX, Magovern CJ, Mack CA, Budenbender KT, Ko W, Rosengart TK. Vascular endothelial growth factor is the major angiogenic factor in the omentum: mechanism of the omentum-mediated angiogenesis. J Surg Res. 1997;67(2):147-154.

Cho CH, Koh J, Han J, Sung HK, Lee HJ, Morisada T, Koh GY. Angiogenic role of LYVE-1-positive macrophages in adipose tissue. Circ Res. 2007;100(4):e47-e57.

Coppack SW. Pro-inflammatory cytokines and adipose tissue. Proc Nutr Soc. 2001;60(3):349-356.

Lijnen HR, Christiaens V, Scroyen I, Voros G, Tjwa M, Carmeliet P, Collen D. Impaired adipose tissue development in mice with inactivation of placental growth factor function. Diabetes. 2006;55(10):2698-2704.

Mu H, Ohashi R, Yan S, Chai H, Yang H, Lin P, Chen C. Adipokine resistin promotes angiogenesis of human endothelial cells in vitro. Cardiovasc Res. 2006;70(1):146-157.

Han RN, Post M, Tanswell AK, Lye SJ. Insulin-like growth factor-I receptor-mediated vasculogenesis/angiogenesis in human lung development. Am J Respir Cell Mol Biol. 2003;28(2):159-169.

Olszanecka-Glinianowicz M, Zahorska-Markiewicz B, Zurakowski A, Glinianowicz M. The role of tumor necrosis factor (TNF-alpha) in control of metabolism. Wiad Lek. 2005;58(11-12):670-674.

Shoshani O, Livne E, Armoni M, Shupak A, Berger J, Ramon Y, Ullmann Y. The effect of interleukin-8 on the viability of injected adipose tissue in nude mice. Plast Reconstr Surg. 2005;115(3):853-859.

Cardinault N, Tourniaire F, Astier J, Couturier C, Bonnet L, Seipelt E, Karkeni E, Letullier C, Dlalah N, Georgé S, Mounien L, Landrier JF. Botanic Origin of Propolis Extract Powder Drives Contrasted Impact on Diabesity in High-Fat-Fed Mice. Antioxidants (Basel). 2021;10(411):1-15.

Xue Y, Petrovic N, Cao R, Larsson O, Lim S, Chen S. Hypoxia-independent angiogenesis in adipose tissues during cold acclimation. Cell Metab. 2009;9:99-109.

Wang H, Chen Y, Lu XA, Liu G, Fu Y, Luo Y. Endostatin prevents dietary-induced obesity by inhibiting adipogenesis and angiogenesis. Diabetes. 2015;64(7):2442-2456.

Kitamura H. Effects of Propolis Extract and Propolis-Derived Compounds on Obesity and Diabetes: Knowledge from Cellular and Animal Models. Molecules. 2019;24(23):1-53.

Culum AA, Yurekli M. Adrenomedullin has a role in angiogenic effects of resveratrol in adipose tissues of obese female rats. Mol Biol Rep. 2020;47:1667-1680.

Silici S. Pre-clinical studies on propolis. Erciyes Univ J Inst Sci Technol. 2015;31(3):185-191.