Investigation of angiogenic factors in obese rats exposed to low oxygen pressure

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Published: Mar 21, 2020
DOI: https://doi.org/10.36472/msd.v7i3.360
Keywords:
Hypoxia, Obesity, HIF1-α, MMP-II, Adrenomedullin

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Meral Dağ
Inonu University, Medical School, Turgut Ozal Medical Center, Malatya TR
Muhittin Yürekli
Inonu University, Arts and Science Faculty, Biology Department, Malatya TR

Abstract

Objective: Obesity, which is one of the most important health problems of today's people, remains current due to the risks of illness it brings due to the increase rate in the world.


Material and Methods: Male Sprague Dawley rats were used in our study of obesity. Rats were divided into four groups as standard diet/ normal oxygen, standard diet/low oxygen, high-fat diet/normal oxygen and high-fat diet / low oxygen. For the study, a special cage with a low oxygen level of 17-18% was made in a closed system. After achieving the desired 25% weight increase in obese group rats, blood, liver, lung, white adipose tissue and brown adipose tissue were obtained from the rats. In these tissues, adrenomedullin, hypoxic inducible factor 1-α (HIF1-α) and matrix metalloproteinase-II (MMP-II) levels were measured by ELISA.


Results: According to our results, there was a significant increase in adrenomedullin, HIF1-α and MMP-II in white adipose tissue, and adrenomedullin and MMP-II in brown adipose tissue. It was found that the amount of HIF1-α increased significantly in liver and lung tissues.


Conclusion: According to the metabolic status of adipose tissue, it is thought that the effect of adrenomedullin, HIF1-α and MMP-II can increase vascularization of brown adipose tissue and provide energy consumption.

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How to Cite
Dağ, M., & Yürekli, M. (2020). Investigation of angiogenic factors in obese rats exposed to low oxygen pressure . Medical Science and Discovery, 7(3), 426–430. https://doi.org/10.36472/msd.v7i3.360
Issue
Vol. 7 No. 3 (2020): Medical Science and Discovery
Section
Research Article

https://creativecommons.org/licenses/by-nc/4.0/

Received 2020-02-13
Accepted 2020-03-13
Published 2020-03-21

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