Effects of Fluoride Exposure on Thiol/ Disulphide Homeostasis, Testicular Oxidative Stress and Histopathological Changes in Rats Effect of fluoride on testicular tissue.

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Yilmaz Kocak
Gokhan Oto
Volkan Kosal
Fikret Altindag
Ahmet Ufuk Komuroglu
Ezgi Onay
Rezzan Temelli Goceroglu


Objective: This study investigated the effects of fluoride-induced toxicity on the reproductive system. For this purpose, testicular oxidative stress, thiol/disulpide homeostasis, sperm motility, density, and histopathology of testicular tissue were evaluated.

Material and Methods: In the experiment, 4 groups were formed, with eight animals (n=8) in each group. Group 1, control/saline, groups 2, 3, and 4 were administered orally sodium fluoride (NaF) at doses of 25, 50, and 100 ppm, respectively, for 30 days. Then the rats were sacrificed and testicular tissue samples were evaluated by sperm examination and histopathologically. Biochemical parameters malondialdehyde (MDA), catalase (CAT), advanced oxidation, and protein products (AOPP) were measured spectrometrically. Thiol/disulphide homeostasis was studied with a fully automated analysis method.

Results: NaF exposure increased MDA and AOPP levels and decreased CAT levels. Disrupted thiol/disulphide homeostasis. Histopathological examination revealed integrity between germinal cells and tubular atrophy. NaF showed reduced sperm motility and density and increased abnormal sperm count. The findings of the NaF 100 ppm group were statistically significant when compared with the Control and other NaF groups (p˂0.05).

Conclusion: The findings concluded that fluoride might cause pathological conditions and infertility in testicular tissue.


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Kocak, Y., Oto, G. ., Kosal, V., Altindag, F., Komuroglu, A. U. ., Onay, E., & Temelli Goceroglu, R. (2023). Effects of Fluoride Exposure on Thiol/ Disulphide Homeostasis, Testicular Oxidative Stress and Histopathological Changes in Rats: Effect of fluoride on testicular tissue. Medical Science and Discovery, 10(3), 130–135. https://doi.org/10.36472/msd.v10i3.904
Research Article
Author Biographies

Gokhan Oto, Dept of Pharmacology, Faculty of Medicine, Yüzüncü Yıl University, Van, TR



Volkan Kosal, Dept of Reproduction and Artificial Insemination, Faculty of Veterinary, Yüzüncü Yıl University, Van, TR



Fikret Altindag, Dept of Histology and Embryology, Faculty of Medicine, Yüzüncü Yıl University, Van, TR



Ezgi Onay, Dept of Pharmacology, Faculty of Medicine, Yüzüncü Yıl University, Van, TR



Rezzan Temelli Goceroglu, Dept of Pharmacology, Faculty of Medicine, Yüzüncü Yıl University, Van, TR



Received 2023-03-01
Accepted 2023-03-09
Published 2023-03-11


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