Dynamic thiol/disulfide homeostasis in gestational diabetes mellitus: Is it related with adverse perinatal outcomes? Thiol/Disulfide homeostasis & gestational diabetes

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Beril Gürlek
Murat Alan
Sabri Çolak
Özgür Önal
Özcan Erel
Cemile Biçer


Objective: Aims of the study is to specify the significance of thiol/disulfide homeostasis in the aspect of gestational diabetes mellitus (GDM) and GDM-related complications.

Material and Methods: This study is a prospective review of the data of 61 healthy and non-pregnant women, 58 healthy pregnant women, and 62 pregnant women with GDM.

Results: The patients with gestational diabetes mellitus had significantly higher disulfide/native thiol and disulfide/total thiol concentrations than non-pregnant patients (p<0.001 for both) and healthy pregnant patients (p: 0.015 and p: 0.018, respectively). Besides, in GDM group had significantly lower native thiol/total thiol concentrations than non-pregnant patients and healthy pregnant patients (p<0.001 and p: 0.016, respectively). There were positive and significant correlations between disulfide levels and HbA1c concentrations (r=0.26, p: 0.042), and between disulfide and oral glucose tolerance test first hour concentrations (r=0.26, p: 0.039). The receiver operating characteristic curve analyses for native thiol, total thiol, and disulfide were unable to predict adverse perinatal outcomes in this cohort.

Conclusion: The significantly higher concentrations of disulfide/native thiol and disulfide/total thiol in women with GDM could be considered as the presence of increased oxidative stress.  However, these markers failed to predict adverse perinatal outcomes.

Keywords: gestational diabetes mellitus; oxidative stress; perinatal outcome; pregnancy; thiol/disulfide homeostasis


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Gürlek, B., Alan, M., Çolak, S., Önal, Özgür, Erel, Özcan, & Biçer, C. (2019). Dynamic thiol/disulfide homeostasis in gestational diabetes mellitus: Is it related with adverse perinatal outcomes? Thiol/Disulfide homeostasis & gestational diabetes. Medical Science and Discovery, 6(9), 198–204. https://doi.org/10.36472/msd.v6i9.293
Research Article


American Diabetes Association. 2. Classification and Diagnosis of Diabetes: Standards of Medical Care in Diabetes-2018. Diabetes Care 2018;41:13-27.

American Diabetes Association. Gestational diabetes mellitus. Diabetes Care 2003;26(suppl 1):103-5.

Practice Bulletin No. 137: Gestational diabetes mellitus. Obstet Gynecol 2013;122(2 Pt 1):406-16.

Yu ZB, Han SP, Zhu GZ, et al. Birth weight and subsequent risk of obesity: A systematic review and meta-analysis. Obes Rev 2011;12:525-42.

Metzger BE, Gabbe SG, Persson B, et al. International association of diabetes and pregnancy study groups recommendations on the diagnosis and classification of hyperglycemia in pregnancy. Diabetes care 2010;33(3):676-82.

Getahun D, Nath C, Ananth CV, Chavez MR, Smulian JC: Gestational diabetes in the United States: temporal trends 1989 through 2004. Am J Obstet Gynecol 2008;198(5):521-5.

Metzger BE, Lowe LP, Dyer AR, et al. Hyperglycemia and adverse pregnancy outcomes. N Engl J Med 2008;358(19):1991-2002.

Gürlek B, Kale I. The prevalence of gestational diabetes mellitus who were admitted to a single center private hospital in Rize. JGON 2019;16(1):31-6.

Linnenkamp U, Guariguata L, Beagley J, et al. The IDF Diabetes Atlas methodology for estimating global prevalence of hyperglycaemia in pregnancy. Diabetes Res Clin Pract 2014;103(2):186-96.

Lappas M, Hiden U, Desoye G, et al. The role of oxidative stress in the pathophysiology of gestational diabetes mellitus. Antioxid Redox Signal 2011;15:3061-100.

Ates I, Kaplan M, Inan B, et al. How does thiol/disulfide homeostasis change in prediabetic patients? Diabetes Res Clin Pract 2015;110:166-71.

Ates I, Kaplan M, Yuksel M, et al. Determination of thiol/disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine 2016;51:47-51.

Ozler S, Oztas E, Caglar Turhan A, et al. Thiol/disulfide homeostasis in predicting adverse perinatal outcomes at 24–28 weeks of pregnancy in gestational diabetes. J Matern Neonatal Med 2016;29:3699-705.

Cremers CM, Jakob U. Oxidant sensing by reversible disulfide bond formation. J Biol Chem 2013;288:26489-96.

Schafer FQ, Buettner GR. Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple. Free Radic Biol Med 2011;30(11):1191-212.

Biswas S, Chida AS, Rahman I. Redox modifications of protein-thiols: Emerging roles in cell signaling. Biochem Pharmacol 2006;71(5):551-64.

Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014;47(18):326-32.

Rodrigues F, de Lucca L, Neme WS, et al. Influence of gestational diabetes on the activity of δ-aminolevulinate dehydratase and oxidative stress biomarkers. Redox Rep 2018;23(1):63-7.

Karacay O, Sepici-Dincel A, Karcaaltincaba D, et al. A quantitative evaluation of total antioxidant status and oxidative stress markers in preeclampsia and gestational diabetic patients in 24-36 weeks of gestation. Diabetes Res Clin Prac 2010;89(3):231-8.

Li H, Yin Q, Li N, et al. Plasma markers of oxidative stress in patients with gestational diabetes mellitus in the second and third trimester. Obstet Gynecol Int 2016;2016:3865454.

Yıldırım M, Türkyılmaz E, Demir-Cendek B, et al. Altered maternal serum dynamic thiol-disulfide interchange reactions in pregnant women with gestational diabetes mellitus. Gynecol Obstet Reprod Med 2016;22(3):129-34.

Roebuck KA. Oxidant stress regulation of IL-8 and ICAM1 gene expression: differential activation and binding of the transcription factors AP-1 and NF-kappaB. Int J Mol Med 1999;4(3):223-30.

López-Tinoco C, Roca M, García-Valero A, et al. Oxidative stress and antioxidant status in patients with late-onset gestational diabetes mellitus. Acta Diabetol 2013;50:201-8.

Zand H, Morshedzadeh N, Naghashian F. Signaling pathways linking inflammation to insulin resistance. Diabetes Metab Syndr 2017;11(1):307-9.

Schmidt MI, Duncan BB, Sharrett AR, et al. Markers of inflammation and prediction of diabetes mellitus in adults (Atherosclerosis Risk in Communities study): a cohort study. Lancet 1999;353:1649-52.

Rueangdetnarong H, Sekararithi R, Jaiwongkam T, et al. Comparisons of the oxidative stress biomarkers concentrations in gestational diabetes mellitus (GDM) and non-GDM among Thai population: cohort study. Endocr Connect 2018;7(5):681-7.